Publication (Embryogensis)(2002-2006）

A. Kikuchi, N. Sanuki, K. Higashi, T. Koshiba and H. Kamada: Abscisic acid and stress treatment are essential for the acquisition of embryogenic competence by carrot somatic cells. Planta, 223; 637-645 (2006)

M. Ikeda, M. Umehara and H. Kamada: Embryogenesis-related genes ; Its expression and roles during somatic and zygotic embryogenesis in carrot and Arabidopsis. Plant Biotechnol., 23; 153-161 (2006)

Y. Ogata, M. Iizuka, D. Nakayama, M. Ikeda, H. Kamada and T. Koshiba: Possible involvement of abscisic acid in the induction of secondary somatic embryogenesis on seed coat-derived carrot somatic embryos. Planta, 221; 417-423 (2005)

M. Umehara, S. Ogita, H. Sasamoto, H. Koshino, T. Asami, S. Fujioka, S. Yoshida and H. Kamada: Identification of a novel factor, vanillyl benzyl ether, which inhibits somatic embryogenesis of Japanese larch (Larix leptolepis Gordon). Plant Cell Physiol., 46(3); 445-453 (2005)

M. Umehara, S. Ogita, H. Sasamoto, C.-H. Eun, Y. Matsubayashi, Y. Sakagami and H. Kamada: Two stimulatory effects of the peptidyl growth factor phytosulfokine during somatic embryogenesis in Japanese larch (Larix leptolepis GORDON). Plant Sci., 169；901-907 (2005)

M. Ikeda and H. Kamada: Comparison of molecular mechanisms of somatic and zygotic embryogenesis. Plant Cell Monographs (2); Somatic Embryogenesis. ed. by A. Mujib and J. Samaj. Springer-Verlag Berlin Heidelberg, pp. 51-68 (2005)

M. Umehara and H. Kamada: Development of the embryo proper and the suspensor during plant embryogenesis. Plant Biotechnol., 22(4); 253-260 (2005)

M. Umehara, S. Ogita, H. Sasamoto and H. Kamada: Inhibitory factor(s) of somatic embryogenesis regulated suspensor differentiation in suspension culture of Japanese larch (Larix leptolepis GORDON). Plant Biotechnol., 21(2); 87-94 (2004)

K. Yazawa, K. Takahata and H. Kamada: Isolation of the gene encoding Carrot leafy cotyledon 1 and expression analysis during somatic and zygotic embryogenesis. Plant Physiol. Biochem., 42; 215-223 (2004)

K. Takahata, M. Takeuchi, M. Fujita, J. Azuma, H. Kamada and F. Sato: Isolation of putative glycoprotein gene from early somatic embryo of carrot and its possible involvement in somatic embryo development. Plant Cell Physiol., 45(11); 1658-1668 (2004)

M. Umehara, S. Ogita, H. Sasamoto and H. Kamada: An in vitro culture system used to investigate possible interactions between the embryo proper and the suspensor in embryogenesis in Japanese larch (Larix leptolepis GORDON). Plant Biotechnol., 21(2); 169-171 (2004)

H. Shiota, G. Yang, S. Shen, C. Eun, K. Watabe, I. Tanaka and H. Kamada: Isolation and characterization of six abscisic acid-inducible genes from carrot somatic embryos.Plant Biotechnol., 21(4); 309-314 (2004)

M. Umehara and H. Kamada: Plant growth regulators involving in embryogenesis in seed plants and its physiological function. Plant Growth Regulation, 39 (1); 42-49 (2004) (in Japanese)

M. Ikeda-Iwai, M. Umehara, S. Satoh and H. Kamada: Stress-induced somatic embryogenesis in vegetative tissues of Arabidopsis thaliana. Plant J., 34；107-114 (2003)

C. Eun, S. Ko, K. Higashi, D. Yeo, Y. Matsubayashi, Y. Sakagami and H. Kamada: Phytosulfokine-α, a peptidyl plant growth factor, stimulates cell cycle progression in carrot non-embryogenic cells. Plant Biotechnol., 20(3); 195-205 (2003)

T. Kobayashi, K. Higashi and H. Kamada: 4-Hydroxybenzyl alcohol accumulates in flowers and developing fruits of carrot and inhibits seed formation. J. Plant Physiol., 160; 713-716 (2003)

C. Eun, S. Ko, Y. Matsubayashi, Y. Sakagami and H. Kamada: Phytosulfokine-α requires auxin to stimulate carrot non-embryogenic cell proliferation.Plant Physiol. Biochem., 41; 447-452 (2003)

W. Mitsuhashi, T. Toyomasu, H. Masui, T. Katho, K. Nakaminami, Y. Kashiwagi, M. Akutsu, H. Kenmoku, T. Sassa, S. Yamaguchi, Y. Kamiya and H. Kamada: Gibberellin is essentially required for carrot (Daucus carota L.) somatic embryogenesis : Dynamic regulation of gibberellin 3-oxidase gene expressions. Biosci. Biochem. Biotechonol., 67(11); 2438-2447 (2003)

M. Ikeda-Iwai, S. Satoh and H. Kamada: Establishment of a reproducible tissue culture system for the induction of Arabidopsis somatic embryos. J. Exp. Bot., 53; 1575-1580 (2002)

M. Umehara, T. Kobayashi, C. Eun, K. Higashi, H. Hanai, Y. Matsubayashi, Y. Sakagami and H. Kamada: Stimulatory and inhibitory conditioning factors regulating somatic embryogenesis in carrot : Recent advances. In Agrobiotechnology and Plant Tissue Culture, eds. by S. S. Bohjwani and W.-Y. Soh, Science Publishers, Inc., pp. 25-36 (2003)

S. Ko and H. Kamada: Motif X and motif Y of the DcECP31 promoter have different functions in the ABA-response pathway. J. Plant Physiol., 159; 1159-1161 (2002)

S. Ko and H. Kamada: Isolation of carrot basic leucine zipper transcription factor using yeast one-hybrid screening. Plant Mol. Biol. Rep., 20; 1-8 (2002)

S. Ko and H. Kamada: Enhancer trapping system for somatic embryogenesis in carrot. Plant Mol. Biol. Rep., 20(4); 421a-421j (2002)

Publication (Flowering)(2002-2006)

T. Mizoguchi, L. Wright, S. Fujiwara, F. Cremer, K. Lee, H. Onouchi, A. Mouradov, S. Fowler, H. Kamada, J. Putterill and G. Coupland: Distinct roles for GIGANTEA in promoting flowering and regulating circadian rhythms in Arabidopsis. Plant Cell, 17; 2255-2270 (2005)

S. Fujiwara, M. Nakagawa, H. Kamada, G. Coupland and T. Mizoguchi: Circadian clock components in Arabidopsis. terminal flower 1 enhances the early flowering phenotype of a mutant, lhy cca1. Plant Biotechnol., 22(4); 311-317 (2005)

S. Fujiwara, A. Oda, H. Kamada, G. Coupland and T. Mizoguchi: Circadian clock components in Arabidopsis. LHY/CCA1 regulate the floral integrator gene SOC1 in both GI-dependent and -independent pathways. Plant Biotechnol., 22(4); 319-325 (2005)

S. Fujiwara, M. Nakagawa, H. Kamada and T. Mizoguchi: Circadian clock components in Arabidopsis . LHY/CCA1/GI in regulating the floral integrator gene LFY/SOC1/FT to control flowering time and shoot architecture. Plant Biotechnol., 22(4); 327-331 (2005)

M. Calvino, H. Kamada and T. Mizoguchi: Is the role of the short-day solely to switch off the CONSTANS in Arabidopsis. Plant Biotechnol., 22(3); 179-183 (2005)

T. Oguchi, K. Sage-Ono, H. Kamada and M. Ono: Genomic structure of a novel Arabidopsis clock-controlled gene, AtC401, which encodes a pentatricopeptide repeat protein. Gene, 330; 29-37 (2004)

T. Oguchi, K. Sage-Ono, H. Kamada and M. Ono: Characterization of transcriptional oscillation of an Arabidopsis homolog of PnC401 related to photoperiodic induction of flowering in Pharbitis nil. Plant Cell Physiol., 45(2); 232-235 (2004)

A. Oda, S. Fujiwara, H. Kamada, G. Coupland and T. Mizoguchi: Antisense suppression of the Arabidopsis PIF3 gene does not affect circadian rhythms but causes early flowering and increases FT expression. FEBS Lett., 557; 259-264 (2004)

Publication (Transgenic plants, Gene literacy education, Collaborative works, others)(2002-2006)

S. Yoshida, M. Tamaoki, T. Shikano, N. Nakajima, D. Ogawa, M. Ioki, M. Aono, A. Kubo, H. Kamada, Y. Inoue and H. Saji: Cytosolic dehydroascorbate reductase is important for ozone tolerance in Arabidopsis thaliana. Plant Cell Physiol., 47(2); 304-308 (2006)

S. Watanabe, T. Sano, H. Kamada and H. Ezura: Reducing gene flow from pollen dispersal of genetically modified plants in special screened greenhouse. Plant Biotechnol., 23; 129-135 (2006)

M. Oto, M. Ono and H. Kamada: Gene literacy education in Japan – Fostering public understanding through practice of hands-on laboratory activities in high schools- . Plant Biotechnol., 23; 339-346 (2006)

K. Takada, K. Ishimaru, H. Kamada and H. Ezura: Anther-specific expression of mutated melon ethylene receptor gene Cm-ERS1/H70A affected tapetum degeneration and pollen grain production in transgenic tobacco plants. Plant Cell Rep., in press (2006)

M. Asahina, Y. Gocho, H. Kamada and S. Satoh: Involvement of inorganic elements in tissue reunion in the hypocotyl cortex of Cucumis sativus. J. Plant Res., 119; 337-342 (2006)

S. Watanabe, H. Kamada and H. Ezura: Effect of a special screened greenhouse covered by fine mesh on maize outcrossing. Plant Biotechnol., 23; 309-316 (2006)

S. Watanabe, T. Sano, H. Kamada and H. Ezura: Efficacy of a special screened greenhouse covered by duplex fine mesh in reducing maize outcrossing. Plant Biotechnol., 23; 387-394 (2006)

B. Ma, M. L. Cui, H. J. Sun, K. Takada, H. Mori, H. Kamada and H. Ezura: Subcellular localization and membrane topology of the melon ethylene receptor CmERS1. Plant Physiol., 141; 587-597 (2006)

K. Ishimaru, K. Takada, S. Watanabe, H. Kamada and H. Ezura: Stable male sterility induced by the expression of mutated melon ethylene receptor genes in Nicotiana tabacum. Plant Sci., 171; 355-359 (2006)

D. Ogawa, N. Nakajima, S. Seo, I. Mitsuhara, H. Kamada and Y. Ohashi: The phenylalanine pathway is the main route of salicylic acid biosynthesis in Tobacco mosaic virus-infected tobacco leaves. Plant Biotechnol., 23; 395-398 (2006)

K. Takada, K. Ishimaru, K. Minamisawa, H. Kamada and H. Ezura: Expression of a mutated melon ethylene receptor gene Cm-ERS1/H69A affects stamen development in Nicotiana tabacum. Plant Sci., 169；935-942 (2005)

H. Inoue, T. Kudo, H. Kamada, M. Kimura, I. Yamaguchi and H. Hamamoto: Copper elicits an increase in cytosolic free calcium in cultured tobacco cells. Plant Physiol. Biochem., 43; 1089-1094 (2005)

M. Ikeda, M. Matsumura and H. Kamada: Suitability of small and branching sunflower varieties for molecular genetic experiments and their transformation by Agrobacterium infection. Plant Biotechnol., 22(2); 97-104 (2005)

D. Ogawa, N. Nakajima, T. Sano, M. Tamaoki, M. Aono, A. Kubo, M. Kanna, M. Ioki, H. Kamada and H. Saji: Salicylic acid accumulation under O3 exposure is regulated by ethylene in tobacco plants. Plant Cell Physiol., 46(7); 1062-1072 (2005)

R. Kikuchi, K. Sage-Ono, H. Kamada and M. Ono: Efficient transformation mediated by Agrobacterium tumefaciens with a ternary plasmid in Pharbitis nil. Plant Biotechnol., 22(4); 295-302 (2005)

K. Sasaki, H. Umetsu, A. Yamada, H. Kamada and Y. Ozeki: Detection of NPT in genetically modified foods with ELISA. Japan. J. Food Chem., 12(3); 140-144 (2005)

T. Ogasawara, Y. Chikagawa, F. Arakawa, A. Nozaki, Y. Itoh, K. Sasaki, H. Umetsu, T. Watanabe, H. Akiyama, T. Maitani, M. Toyoda, H. Kamada, Y. Goda and Y. Ozeki: Frequency of mutations of the transgene, which might result in the loss of the glyphosate-tolerant phenotype, was lowered in Roundup Ready  soybeans. J. Health Sci., 51(2); 197-201 (2005)

D. Ogawa, N. Nakajima, T. Sano, M. Tamaoki, M. Aono, A. Kubo, H. Kamada and H. Saji: Regulation of salicylic acid synthesis in ozone-exposed tobacco and Arabidopsis. PHYTON-ANN REI BOT A., in press (2005)

K. Gomi, D. Ogawa, S. Katou, H. Kamada, N. Nakajima, H. Saji, T. Soyano, M. Sasabe, Y. Machida, I. Mitsuhara, Y. Ohashi and S. Seo: A mitogen-activated protein kinase NtMPK4 activated by SIPKK is required for jasmonic acid signaling and involved in ozone tolerance via stomatal movement in tobacco. Plant Cell Physiol., 46(12); 1902-1914 (2005)

K. Takada, H. Kamada and H. Ezura: Production of male sterile transgenic plants using ethylene receptor genes. Plant Biotechnol., 22(5); 469-476 (2005)

M. Umehara, I. Eguchi, D. Kaneko, M. Ono and H. Kamada: Evaluation of gene flow and its environmental effects in the field. Plant Biotechnol., 22(5); 497-504 (2005)

T. Mizoguchi, A. Kikuchi, T. Kato, H. Kamada and K. Shinozaki: Isolation of Arabidopsis mutants that have altered sensitivity to staurosporine and ABA. Plant Biotechnol., 22(4); 303-310 (2005)

T. Saitou, A. Hashidume, S. Tokutomi and H. Kamada: Reduction of phytochrome level and light-induced formation of adventitious shoots by introduction of antisense genes for phytochrome A in horseradish hairy roots.Plant Cell, Tissue, Organ Culture, 76; 45-51 (2004)

H. Yamakawa, S. Katou, S. Seo, I. Mitsuhara, H. Kamada and Y. Ohashi: Plant MAPK phosphatase interacts with calmodulins. J. Biol. Chem., 279(2); 928-936 (2004)

M. L. Cui, H. Ezura, S. Nishimura, H. Kamada and T. Handa: A rapid Agrobacterium-mediated transformation of Antirrhinum majus L. by using direct shoot regeneration from hypocotyl explants. Plant Sci., 166(4); 873-879 (2004)

K. Yoshimatsu, H. Sudo, H. Kamada, F. Kiuchi, Y. Kikuchi, J. Sawada and K. Shimomura: Tropane alkaloid production and shoot regeneration in hairy and adventitious root cultures of Duboisia myoporoides – D. leichhardtii hybrid. Biol. Pharm. Bull., 27(8); 1261-1265 (2004)

I. Eguchi, M. Umehara, M. Ono and H. Kamada: Proposal to investigate gene flow and gene diversity using carrot (Daucus carota L.). Proc. of Korea Conference on Innovative Science and Technology 2004: GM Crops and Foods -Potential, Safety & Environmental Impact, pp. 69-82 (2004)

H. Yamaguchi, K. Sasaki, H. Umetsu and H. Kamada: Two detection methods of genetically modified maize and the state of its import into Japan. Food Control, 14(3); 201-206 (2003)

R. Kaida, K. Sage-Ono, H. Kamada, H. Okuyama, K. Syono and T. S. Kaneko: Isolation and characterization of four cell wall purple acid phosphatase genes from tobacco cells. Biochim. Biophys. Acta (Gene Structure and Expression), 1625(2); 134-140 (2003)

R. Kato, M. Arashida, M. Kodama, H. Kamada and T. Suzuki: Inhibitions of growth and lateral branch development by calmodulin antagonists in hairy roots of Lithospermum erythrorhizon, Atropa belladonnna and Daucus carota. Bulletin of the Yamagata University, Yamagata, Japan, (Natural Science), Vol.15, No.3; pp. 79-88 (2003)

T. Ogawara, K. Higashi, H. Kamada and H. Ezura: Ethylene advances the transition from vegetative growth to flowering in Arabidopsis thaliana. J. Plant Physiol, 160; 1335-1340 (2003)

T. Saitou, A. Hashidume, K. Takahata, T. Suzuki, S. Tokutomi and H. Kamada: Phytochrome activities and biological activities of genes for phytochrome A of horseradish in horseradish hairy roots.Plant Biotechnol., 20; 13-19 (2003)

A. Oda, C. Sakuta, H. Kamada and S. Satoh: Xylem sap lectin, XSP30, recognizes GlcNAc sugar chains of glycoproteins in cucumber leaves. Plant Biotechnol., 20; 67-74 (2003)

H. Iwai, M. Usui, H. Hoshino, H. Kamada, T. Matsunaga, K. Kakegawa, T. Ishii and S. Satoh: Analysis of sugars in squash xylem sap. Plant Cell Physiol., 44(6); 582-587 (2003)

M. L. Cui, H. Ichikawa, K. Sage-Ono, H. Kamada and T. Handa: Agrobacterium-mediated transformation of Asarina procumbens Mill. J. Hort. Sci. Biotechnol., 78(3); 394-399 (2003)

A.Oda, C. Sakuta, S. Masuda, T. Mizoguchi, H. Kamada and S. Satoh: Possible involvement of leaf gibberellins in the clock-controlled expression of XSP30, a gene encoding a xylem sap lectin, in cucumber roots. Plant Physiol., 133; 1779-1790 (2003)

K. Sasaki, T. Ishise, K. Shimomura, T. Kobayashi, Y. Matsubayashi, Y. Sakagami, H. Umetsu and H. Kamada: Effects of phytosulfokine-α on growth and tropane alkaloid production in Atropa belladonna hairy roots. Plant Growth Regulation, 36; 87-90 (2002)

M. Asahina, H. Iwai, A. Kikuchi, S. Yamaguchi, Y. Kamiya, H. Kamada and S. Satoh: Gibberellin produced in the cotyledon is required for cell division during tissue-reunion in the cortex of cut cucumber and tomato hypocotyls. Plant Physiol., 129; 201-210 (2002)

N. Nakajima, T. Itoh, S. Takikawa, N. Asai, M. Tamaoki, M. Aono, A. Kubo, H. Kamada and H. Saji: Improvement in ozone tolerance of tobacco plants with an antisense DNA for 1-aminocyclopropane-1-caboxylate synthase. Plant Cell Environment, 25; 727-735 (2002)

C. Kato, H. Kato, T. Asami, S. Yoshida, H. Noda, H. Kamada and S. Satoh: Involvement of xylem sap zeatin O-glucoside in the greening of cucumber seedling. Plant Physiol. Biochem., 40; 949-954 (2002)

T. Kuroha, H. Kato, T. Asami, S. Yoshida, H. Kamada and S. Satoh: A trans-zeatin riboside in root xylem sap negatively regulates adventitious root formation on cucumber hypocotyls. J. Exp. Bot., 53; 2193-2200 (2002)

H. Kamada: General consideration of safety assessment of genetically modified foods including concept of substantial equivalence. Abstract of “2002 International Symposium on the Safety Assessment and Regulation for the Genetically Modified Foods”, pp. 65-73 (2002)